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FIGURE2.GRPtissuedistribution(A),sitesofgeneexpression(B),andcorre- spondingquantificationofGRPexpressionlevelspercelltype(C)inAdriatic sturgeon. A, levels of GRP gene expression measured by real-time <t>qPCR</t> in adult tissues related to levels in heart (Ht). Lv, liver; Kd, kidney; Ms, muscle; Gn, gonads; Br, brain; GP, ganoid plate; AK, anterior kidney; Sl, spleen; Sp, spine; Ct, cleithrum; HP, head plate; Op, operculum; Sk, skull; Md, mandibula; BA, branchial arches; AV, anterior vertebra; PV, posterior vertebra. B, sites of gene expression determined by in situ hybridization in vertebra (panels 1 and 2) and mandibula (panel 3). Cb, chordoblastlayerofthenotochord;Cd,chordocytes;NtSh,notochordsheath;Ct, cartilage; IC, immature chondrocytes; MC, mature chondrocytes. Hybridization with sense probe is presented in panel 4. Panels 1, 2, 3, and 4, magnification 20. C, the percentage of IM, MC, and chordoblasts (Cd) expressing GRP was deter- mined by dividing the number of cells of the indicated type expressing GRP by thetotalnumberofcellsofthattypedetectedin3consecutivevertebrasections. p was calculated using the unilateral Student’s t test.
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FIGURE2.GRPtissuedistribution(A),sitesofgeneexpression(B),andcorre- spondingquantificationofGRPexpressionlevelspercelltype(C)inAdriatic sturgeon. A, levels of GRP gene expression measured by real-time <t>qPCR</t> in adult tissues related to levels in heart (Ht). Lv, liver; Kd, kidney; Ms, muscle; Gn, gonads; Br, brain; GP, ganoid plate; AK, anterior kidney; Sl, spleen; Sp, spine; Ct, cleithrum; HP, head plate; Op, operculum; Sk, skull; Md, mandibula; BA, branchial arches; AV, anterior vertebra; PV, posterior vertebra. B, sites of gene expression determined by in situ hybridization in vertebra (panels 1 and 2) and mandibula (panel 3). Cb, chordoblastlayerofthenotochord;Cd,chordocytes;NtSh,notochordsheath;Ct, cartilage; IC, immature chondrocytes; MC, mature chondrocytes. Hybridization with sense probe is presented in panel 4. Panels 1, 2, 3, and 4, magnification 20. C, the percentage of IM, MC, and chordoblasts (Cd) expressing GRP was deter- mined by dividing the number of cells of the indicated type expressing GRP by thetotalnumberofcellsofthattypedetectedin3consecutivevertebrasections. p was calculated using the unilateral Student’s t test.
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FIGURE2.GRPtissuedistribution(A),sitesofgeneexpression(B),andcorre- spondingquantificationofGRPexpressionlevelspercelltype(C)inAdriatic sturgeon. A, levels of GRP gene expression measured by real-time <t>qPCR</t> in adult tissues related to levels in heart (Ht). Lv, liver; Kd, kidney; Ms, muscle; Gn, gonads; Br, brain; GP, ganoid plate; AK, anterior kidney; Sl, spleen; Sp, spine; Ct, cleithrum; HP, head plate; Op, operculum; Sk, skull; Md, mandibula; BA, branchial arches; AV, anterior vertebra; PV, posterior vertebra. B, sites of gene expression determined by in situ hybridization in vertebra (panels 1 and 2) and mandibula (panel 3). Cb, chordoblastlayerofthenotochord;Cd,chordocytes;NtSh,notochordsheath;Ct, cartilage; IC, immature chondrocytes; MC, mature chondrocytes. Hybridization with sense probe is presented in panel 4. Panels 1, 2, 3, and 4, magnification 20. C, the percentage of IM, MC, and chordoblasts (Cd) expressing GRP was deter- mined by dividing the number of cells of the indicated type expressing GRP by thetotalnumberofcellsofthattypedetectedin3consecutivevertebrasections. p was calculated using the unilateral Student’s t test.
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FIGURE2.GRPtissuedistribution(A),sitesofgeneexpression(B),andcorre- spondingquantificationofGRPexpressionlevelspercelltype(C)inAdriatic sturgeon. A, levels of GRP gene expression measured by real-time <t>qPCR</t> in adult tissues related to levels in heart (Ht). Lv, liver; Kd, kidney; Ms, muscle; Gn, gonads; Br, brain; GP, ganoid plate; AK, anterior kidney; Sl, spleen; Sp, spine; Ct, cleithrum; HP, head plate; Op, operculum; Sk, skull; Md, mandibula; BA, branchial arches; AV, anterior vertebra; PV, posterior vertebra. B, sites of gene expression determined by in situ hybridization in vertebra (panels 1 and 2) and mandibula (panel 3). Cb, chordoblastlayerofthenotochord;Cd,chordocytes;NtSh,notochordsheath;Ct, cartilage; IC, immature chondrocytes; MC, mature chondrocytes. Hybridization with sense probe is presented in panel 4. Panels 1, 2, 3, and 4, magnification 20. C, the percentage of IM, MC, and chordoblasts (Cd) expressing GRP was deter- mined by dividing the number of cells of the indicated type expressing GRP by thetotalnumberofcellsofthattypedetectedin3consecutivevertebrasections. p was calculated using the unilateral Student’s t test.
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FIGURE2.GRPtissuedistribution(A),sitesofgeneexpression(B),andcorre- spondingquantificationofGRPexpressionlevelspercelltype(C)inAdriatic sturgeon. A, levels of GRP gene expression measured by real-time <t>qPCR</t> in adult tissues related to levels in heart (Ht). Lv, liver; Kd, kidney; Ms, muscle; Gn, gonads; Br, brain; GP, ganoid plate; AK, anterior kidney; Sl, spleen; Sp, spine; Ct, cleithrum; HP, head plate; Op, operculum; Sk, skull; Md, mandibula; BA, branchial arches; AV, anterior vertebra; PV, posterior vertebra. B, sites of gene expression determined by in situ hybridization in vertebra (panels 1 and 2) and mandibula (panel 3). Cb, chordoblastlayerofthenotochord;Cd,chordocytes;NtSh,notochordsheath;Ct, cartilage; IC, immature chondrocytes; MC, mature chondrocytes. Hybridization with sense probe is presented in panel 4. Panels 1, 2, 3, and 4, magnification 20. C, the percentage of IM, MC, and chordoblasts (Cd) expressing GRP was deter- mined by dividing the number of cells of the indicated type expressing GRP by thetotalnumberofcellsofthattypedetectedin3consecutivevertebrasections. p was calculated using the unilateral Student’s t test.
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FIGURE2.GRPtissuedistribution(A),sitesofgeneexpression(B),andcorre- spondingquantificationofGRPexpressionlevelspercelltype(C)inAdriatic sturgeon. A, levels of GRP gene expression measured by real-time <t>qPCR</t> in adult tissues related to levels in heart (Ht). Lv, liver; Kd, kidney; Ms, muscle; Gn, gonads; Br, brain; GP, ganoid plate; AK, anterior kidney; Sl, spleen; Sp, spine; Ct, cleithrum; HP, head plate; Op, operculum; Sk, skull; Md, mandibula; BA, branchial arches; AV, anterior vertebra; PV, posterior vertebra. B, sites of gene expression determined by in situ hybridization in vertebra (panels 1 and 2) and mandibula (panel 3). Cb, chordoblastlayerofthenotochord;Cd,chordocytes;NtSh,notochordsheath;Ct, cartilage; IC, immature chondrocytes; MC, mature chondrocytes. Hybridization with sense probe is presented in panel 4. Panels 1, 2, 3, and 4, magnification 20. C, the percentage of IM, MC, and chordoblasts (Cd) expressing GRP was deter- mined by dividing the number of cells of the indicated type expressing GRP by thetotalnumberofcellsofthattypedetectedin3consecutivevertebrasections. p was calculated using the unilateral Student’s t test.
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FIGURE2.GRPtissuedistribution(A),sitesofgeneexpression(B),andcorre- spondingquantificationofGRPexpressionlevelspercelltype(C)inAdriatic sturgeon. A, levels of GRP gene expression measured by real-time qPCR in adult tissues related to levels in heart (Ht). Lv, liver; Kd, kidney; Ms, muscle; Gn, gonads; Br, brain; GP, ganoid plate; AK, anterior kidney; Sl, spleen; Sp, spine; Ct, cleithrum; HP, head plate; Op, operculum; Sk, skull; Md, mandibula; BA, branchial arches; AV, anterior vertebra; PV, posterior vertebra. B, sites of gene expression determined by in situ hybridization in vertebra (panels 1 and 2) and mandibula (panel 3). Cb, chordoblastlayerofthenotochord;Cd,chordocytes;NtSh,notochordsheath;Ct, cartilage; IC, immature chondrocytes; MC, mature chondrocytes. Hybridization with sense probe is presented in panel 4. Panels 1, 2, 3, and 4, magnification 20. C, the percentage of IM, MC, and chordoblasts (Cd) expressing GRP was deter- mined by dividing the number of cells of the indicated type expressing GRP by thetotalnumberofcellsofthattypedetectedin3consecutivevertebrasections. p was calculated using the unilateral Student’s t test.

Journal: Journal of Biological Chemistry

Article Title: Gla-rich Protein (GRP), A New Vitamin K-dependent Protein Identified from Sturgeon Cartilage and Highly Conserved in Vertebrates

doi: 10.1074/jbc.m802761200

Figure Lengend Snippet: FIGURE2.GRPtissuedistribution(A),sitesofgeneexpression(B),andcorre- spondingquantificationofGRPexpressionlevelspercelltype(C)inAdriatic sturgeon. A, levels of GRP gene expression measured by real-time qPCR in adult tissues related to levels in heart (Ht). Lv, liver; Kd, kidney; Ms, muscle; Gn, gonads; Br, brain; GP, ganoid plate; AK, anterior kidney; Sl, spleen; Sp, spine; Ct, cleithrum; HP, head plate; Op, operculum; Sk, skull; Md, mandibula; BA, branchial arches; AV, anterior vertebra; PV, posterior vertebra. B, sites of gene expression determined by in situ hybridization in vertebra (panels 1 and 2) and mandibula (panel 3). Cb, chordoblastlayerofthenotochord;Cd,chordocytes;NtSh,notochordsheath;Ct, cartilage; IC, immature chondrocytes; MC, mature chondrocytes. Hybridization with sense probe is presented in panel 4. Panels 1, 2, 3, and 4, magnification 20. C, the percentage of IM, MC, and chordoblasts (Cd) expressing GRP was deter- mined by dividing the number of cells of the indicated type expressing GRP by thetotalnumberofcellsofthattypedetectedin3consecutivevertebrasections. p was calculated using the unilateral Student’s t test.

Article Snippet: PCR, set up in duplicates, were as follows: 2 l of cDNA (from reverse transcriptase diluted 1:10), 8 l of primermixture (each primer at 0.2 M final concentration), and 10 l of Absolute QPCR SYBR Green Fluorescein mixture (ABgene, Epsom, UK).

Techniques: Gene Expression, In Situ Hybridization, Hybridization, Expressing